Pharmacognostical and phytochemical evaluation of leaves extract. Pdf phytochemical screening and antimicrobial activity of leaf. It is originally native to the tropical areas of the americas from mexico to argentina, and has been spread throughout the world in tropical and subtropical regions around the world, becoming naturalized or invasive in many areas. Phytochemical screening and wound healing activities of. Two of them, 2 and 3, are of lathyrane type while the other two, 4 and. Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil and presscake of jatropha curcas amit kumar sharma, mayank gangwar, dharmendra kumar, gopal nath, akhoury sudhir kumar sinha, yamini bhushan tripathi. Acute toxicity studies were done by using female albino mice weighing 2335 gm as per oecd 425 guidelines. Pdf in vitro propagation and phytochemical screening of. Phytochemical studies were carried out on the leaf, stem, root and seed of four jatropha l. Pdf on jan 10, 2016, m oyama and others published phytochemical screening and antimicrobial activity of leaf extract of jatropha curcas find, read and cite all the research you need on.
In vitro propagation and phytochemical screening of jatropha. In vitro antibacterial activity and phytochemical screening. Phytochemical characterization, antimicrobial activity and reducing. Phytochemical evaluation and antioxidant study of jatropha curcas seeds. Phytochemical and antibacterial studies on jatropha curca s l. Jatropha curcas is an ornamental plant which is also employed to cure various infections in traditional medicine. Pdf jatropha is a large genus comprising over 170 species. The preliminary phytochemical screening was carried out in ethyl acetate extract of jatropha gossypifolia by following the method of harbone 9. In vitro propagation and phytochemical screening of jatropha curcas shobha gawri, ajay singh, prashant shukla and arunima upadhyay g. Present study includes physicochemical, phytochemical, antioxidant and hptlc analysis of j. In vitro antibacterial activity and phytochemical screening of crude extracts from jatropha curcas linn k. Comparative phytochemical and antimicrobial screening of. Euphorbiaceae leaves extract vidhya kumari, siddhi gupta, nitu kumari, dr.
The phytochemical analysis of the methanol extract of the leaf, stem bark and root of. The in vitro antimicrobial activity of crude ethanolic, methanolic and water extracts of the stem bark of jatropha curcas were investigated. Jatropha curcas shows invasive characters and is a significant source of many phytochemicals with varying biological activities. Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents m madhu, v sailaja, tnvss satyadev, mv satyanarayana abstract. The uses of various parts of jatropha plant are well known from ages. Aug 25, 2017 antimicrobial activity and phytochemical screening of stem bark extract of jatropha curcas. In vitro antimicrobial activity and phytochemical screening. Euphorbiaceae leaf extract was evaluated against one grampositive species. In vitro propagation and phytochemical screening of jatropha curcas. The structure of jatrophenone 1 was established mainly by detailed studies of its 1d and 2d nmr. Phytochemical and biological evaluation of defatted seeds. Leaves and ovary walls were found to contain higher contents of total phenols, tannins and phytic acid whereas free amino acids were greater in leaves.
As a measure of testing the medicinal properties of jatropha curcas, methanol extract obtained from both in vivo leaf and leaf derived callus were subjected to antimicrobial activity against six microorganisms, of the six different concentrations tested, the in vitro leaf callus extracts of at high concentrations 1. Phytochemical and antibacterial property of crude petroleum ether, chloroform, acetone, methanolic and aqueous extracts of jatropha curcas l leaves were studied as part of searching new bioactive. The presence of phenolic compounds was identified by taking 1 ml of extract with 5 ml alcohol and a pinch of ferric chloride. Phytochemical analysis of jatropha gossypifolia linn. In vitro antimicrobial activity and phytochemical screening of jatropha curcas seed extract daniyan s. Acute toxicity studies were done by using female albino mice weighing 2335 gm as. Phytochemical screening revealed the presence of bioactive compound saponin in all the parts of the three plants in water extract only. The various extract of leaves jatropha curcas linn. Jun 03, 2015 phytochemical screening of the extracts of the root and the latex of jatropha curcas the methods described by odebiyi and sofowora 1978 were used to test for the presence of saponins, tannins, phenolics and alkaloids, lieberman burchad reaction as described by herburne 1973 was used to test for steroids, while the salkowski test was used to. A voucher specimen 262541 has been submitted in institutes herbarium. The analgesic and antiinflammatory effects of the methanolic extract of the leaves of jatropha curcas were investigated in mice and rats respectively. Pdf on jan 10, 2016, m oyama and others published phytochemical screening and antimicrobial activity of leaf extract of jatropha curcas.
Present study investigated the phytochemical components and antimicrobial activities of jatropha caurcas against some selected clinical isolates. Accepted 23 may, 2014 phytochemical studies were carried out on the plant parts of. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 5 to 12, 8 to 20 and 0 to 8 mm for ethanol, methanol and water extracts respectively. The analgesic effect was determined by acetic acid induced writhing test in mice. Phytochemistry and antimicrobial assay of jatropha curcas extracts. Jatropha curcas euphorbiaceae is a multipurpose plant with many attributes and considerable potential. In many subtropical and semiarid regions, traditionally, j.
Jatropha curcas, euphorbiaceae, hptlc, pharmacognostical, phytochemical analysis. The phytochemical screening of the aqueous, ethanolic and methanolic extracts of the j. Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of rumex dentatus l. Phytochemical profiling of phytoconstituents of grape. Pdf phytochemical screening and antimicrobial activity of. An value added herbal drug paperback february 6, 2011 by akshada kakade author, c. Pdf antimicrobial activity and phytochemical screening. The methanolic leaf extract of the plant revealed the presence of bioactive compounds like flavonoids, tannins. Jatropha curcas is a species of the genus jatropha, belonging to the family euphorbiaceae. Saponin was absent in other solvents used ethanol, ethyl acetate, propan2ol, methanol. Antimicrobial activity and phytochemical screening of stem bark extract of jatropha curcas. In vitro antibacterial activity and phytochemical screening of crude extracts from jatropha curcas linn. Phytochemical screening and antimicrobial activities of jatropha curcas l. The phytochemical screening for the methanolic extract revealed the presence of alkaloids, tannins, saponins, flavonoids and cardiac glycosides while the ethanolic anthraquninones and combined anthraquinones.
Acute and chronic toxicity studies of tubatuba jatropha. The phytochemical screening of the oil showed the presence of saponins, alkaloids, steroids, terpenoids and cardiac glycosides, while tannins, flavonoids and phenols were absent. Phytochemical screening was done by standard procedures. Jatropha curcas is a plant that produces high oil content seeds used. Different plant parts of jatropha curcas l exhibited variation in their phytochemical constituents. Phytochemical analysis of various plant parts of jatropha curcas revealed variation in contents of total phenols, tannins, free amino acids and phytic acid.
Department of microbiology, federal university of technology, minna niger state, nigeria article received on. S 1 department of pharmacognosy and ethnotherapy universitty of port harcourt 2 department ofpharmacology university of port harcourt rivers state nigeria. Pdf phytochemical screening and antimicrobial activity. From the determined parameters, jatropha curcas seed oil may be use as cooking oil and in cosmetics. The first compound is a novel diterpene while the other was isolated from the species for the first time. This study investigated the phytoconstituents in neem seed and flower, jatropha curcas stem and root bark and grape stem bark and leaves in some selected solvents. Phytochemical screening and antimicrobial activities of. Jatropha curcas, nhexane, ethyl acetate, traditional medicine, phytochemical. Qualitative result revealed the presence of tannin, saponin, alkaloid, total. The results suggest that the phytochemicals properties of the seed for curing various ailments.
Euphorbiaceae, widely known as bellyache bush, is a medicinal plant largely used throughout africa and america. Phytochemical evaluation and antioxidant study of jatropha. Phytochemical and antibacterial property of crude petroleum ether, chloroform, acetone, methanolic and aqueous extracts of jatropha curcas l leaves were studied as part of searching new bioactive compounds. The phytochemical constituents, analgesic and antiinflammatory effects of methanol extract of jatropha curcas leaves in mice and wister albino rats. Both quantitative and qualitative phytochemical screening was carried out on stems, seeds and leaves of jatropha from forest and guinea savannah.
An value added herbal drug kakade, akshada, magdum, c. Preliminary phytochemical analysis qualitative phytochemical analysis of the crude powder of the leaves collected was determined according to the standard procedures to identify the constituents as described by 1417. International journal of current microbiology and applied. The leaves extract of jatropha curcas in chloroform exhibited the presence of alkaloides, cardiac glycosides and steroids. Scholars research library extraction, physicochemical. Analysis of the phytochemical content and antimicrobial. It is a plant that produces seeds with high oil content that can be used as biodiesel. Jatropha curcas seed collected in june 2008 from banthra research station lucknow, india. Several human and veterinary uses in traditional medicine are described for different parts and preparations based on this plant. Pdf in vitro antimicrobial activity and phytochemical. A comparison of pergularia daemia and jatropha curcas leaf extracts. In vitro antimicrobial activity and phytochemical analysis of.
Pdf jatropha curcas shows invasive characters and is a significant source of many phytochemicals with varying biological activities. Qualitative and quantitative analysis of five secondary metabolites alkaloids, tannins. Page 117 arkat usa, inc from jatropha gossypifolia two macrocyclic diterpenes, jatrophenone 112 and citlalitrione 1520 were isolated. The phytochemical screening of the extract was also carried out.
Herbal ointment, jatropha curcas, phytochemical screening, wound. The oil from seeds is used in relieving rashes and parasitic skin. The minimum inhibitory concentration mic of the ethanol extract was between 0. Issn 22293566 phytochemical screening of leaves of jatropha curcas plant ahirrao r.
Phytochemical analysis and antimicrobial screening of jatropha curcas were carried out on forest and guinea savannah samples. Pdf antimicrobial activity and phytochemical screening of. Comparative study of the phytochemical properties of jatropha. Pdf physicochemical characterization and phytochemical. Physicochemical characterization and phytochemical screening of. While the anti inflammatory activity was determined by egg albumin induced oedema of the. Comparative study of the phytochemical properties of. Leaves and ovary walls contain the maximum contents of phenols, tannins and phytic acid whereas, free amino acids were greater in leaves and seeds fig. Physicochemical characterization and phytochemical screening.
Antimicrobial activity of the biodiesel plant, jatropha. Phytochemical analysis and preliminary screening of antimicrobial activity of jatropha curcas l abhijit b shinde abstract in the present study, the antimicrobial activity of jatropha curcas l. The hexane, ethyl acetate and methanol extracts of this plant were analysed phytochemically and screened against different microorganisms responsible for various infections especially sexually transmitted diseases. Previous researchers studies different parts of jatropha plant and reported that leaf extract of jatrophacurcas has significant wound healing activity shetty et al. The phytochemical constituents, analgesic and anti. Phytochemical screening one milliliter of the waterfree and ethanolfree extract solution were tested for the presence of deoxysugars, tannins, alkaloids, sterols, flavonoids, unsaturated lactones. Jatropha caurcas euphorbiaceae is used in folklore medicine to cure various ailments in africa, asia and latin america. Comparative study of the phytochemical properties of jatropha curcas and azadirachta indica plant extracts harryasobara, joy linda and samson, enoobong okon forestry research institute of nigeria, eastern research station, umuahia, abia state, nigeria. Jatropha curcas linn were carried out using standard methods and revealed. Sharma 1,3, mohammed ali 2, harneet singh 1, shahnaz sultana 2,4, showkat r. Phytochemical and biological evaluation of defatted seeds of.
The study includes preparation of different extracts by successive solvent extraction for detailed analysis. Antibacterial activity the following bacterial strains escherichia coli, proteus sp. Physicochemical characterization and phytochemical screening of jatropha. Qualitative phytochemistry and antibacterial resistance. Experimental section collection of materials jatropha curcas l. Phytochemical and biological evaluation of defatted seeds of jatropha curcas penilaian fitokimia dan biologi biji jatropha curcas nyahlemak m uhammad n isar u l h aq, s ultan m ehmood w azir, f aizan u llah, r ahmat a li k han. Penilaian fitokimia dan biologi biji jatropha curcas nyahlemak. The present study was carried on methanolic extracts of four jatropha species leaves to investigate the presence of medicinally important phytochemicals in the leaves of different jatropha species. Phytochemical screening the phytochemical analysis was done according to the method reported by 10,11,12. Qualitative phytochemical screening of jatrophacurcas extract.
Euphorbiaceae leaf extract was evaluated against one grampositive species staphylococcus aureus, and four gramnegative species. Preliminary phytochemical screening was performed as per standardized procedure of the various phytoconstituents in methanol, chloroform and water extract. Assessment of phytochemical and antimicrobial screening of. Investigation on jatropha curcas yielded a large number of diterpenes table 1. The seeds are not edible and contain phorbol ester that is toxic3,4. Ash value total ash, acid insoluble ash and water soluble ash, extractive values, loss on drying were studied dry weight. Pdf the antimicrobial effects of the methanol, ethyl acetate and hexane extracts of j. Phytochemical investigation of the roots of jatropha curcas l. Jatropha curcas, antimicrobial activity, phytochemical screening, minimum inhibitory concentration. Rungta college of science and technology, kohkakurud road, bhilai490 024, chhattisgarh, india corresponding author. Physicochemical characterization and phytochemical. With this knowledge, the present study was aimed to study the phytochemical and antibacterial property of crude extracts of j. In vitro antimicrobial activity and phytochemical analysis.